Titelaufnahme

Titel
Neuronal network activity of nucleus accumbens and septal nuclei in C57BL/6 mice recorded using multi-electrode arrays / by Kai Kummer
VerfasserKummer, Kai
Begutachter / BegutachterinKlimaschewski, Lars ; Lawrence, Andrew
Betreuer / BetreuerinSaria, Alois
Erschienen2014
Umfang88 Bl. : Ill., graph. Darst.
HochschulschriftInnsbruck, Med. Univ., Diss., 2014
Anmerkung
Enth. u.a. 2 Veröff. d. Verf. aus den Jahren 2014
Datum der AbgabeJanuar 2015
SpracheEnglisch
Bibl. ReferenzOeBB
DokumenttypDissertation
Schlagwörter (DE)cocaine CPP / social interaction CPP / multi-electrode array / spontaneous activity / spike frequency / nucleus accumbens / septum
Schlagwörter (GND)Cocain / Interaktion / Konditionierung / Mehrfachelektrode / Tiermodell
Zugriffsbeschränkung
 Das Dokument ist ausschließlich in gedruckter Form in der Bibliothek vorhanden.
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Zusammenfassung (Deutsch)

We previously developed a rat experimental model based on the conditioned place preference (CPP) paradigm in which only four 15-min episodes of dyadic social interaction with a sex- and weight-matched male Sprague Dawley rat (1) reversed CPP from cocaine to social interaction despite continuing cocaine training, and (2) prevented the reacquisition/re-expression of cocaine CPP. In a concurrent conditioning schedule, pairing one compartment with social interaction and the other compartment with 15 mg/kg cocaine injections, rats spent the same amount of time in both compartments. Both cocaine and social interaction conditioning led to increased neuronal expression of the immediate early gene EGR1 in the nucleus accumbens and other reward associated brain regions, suggesting that both drug- and natural rewards may be processed in similar brain regions.

One aim of the present thesis was to validate our experimental paradigm in C57BL/6 mice to investigate if our experimental paradigm may be useful for the considerable number of genetically modified mouse models. Only 71% of the tested mice developed place preference for social interaction, whereas 85% of the rats did. In support of the lesser likelihood of mice to develop a preference for social interaction, the average amount of time spent in direct contact was 17% for mice vs. 79% for rats. In animals that were concurrently conditioned for social interaction vs. cocaine, the relative reward strength for cocaine was 300-fold higher in mice than in rats. Considering that human addicts regularly prefer drugs of abuse to drug-free social interaction, the present findings suggest that our experimental paradigm of concurrent CPP for social interaction vs. cocaine is of even greater translational power if performed in C57BL/6 mice, the genetic background for most transgenic rodent models, than in rats.

In order to gain novel insights into the intrinsic in vitro electrical activity of the nucleus accumbens and adjacent brain regions and to explore the effects of reward conditioning on network activity, we performed multi-electrode array recordings of spontaneous firing in acute brain slices of mice conditioned for either cocaine- or social interaction CPP. Cocaine conditioning increased the spike frequency of neurons in the septal nuclei, whereas social interaction conditioning increased the spike frequency in the nucleus accumbens, compared to saline control animals. In addition, social interaction conditioning decreased the amount of active neuron clusters in the nucleus accumbens. Our findings suggest that place preference conditioning for both drug and natural rewards may induce persistent changes in neuronal network activity in the accumbens and the septum that are still preserved in acute slice preparations.